This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces
cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.
This method is applicable to:
— fresh water;
— wastewater;
— aqueous extracts and leachates;
— eluates of sediments (fresh water);
— pore water;
— aqueous solutions of single substances or of chemical mixtures;
— drinking water.
The limit of quantification (LOQ) of this method for the direct analysis of water samples is between
8 ng/l and 15 ng/l 17β‐estradiol equivalents (EEQ) based on the results of the international
interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between
120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above
this threshold have to be diluted for a valid quantification. Extraction and pre‐concentration of water
samples can prove necessary, if their estrogenic potential is below the given LOQ.
Registration number (WIID)
73875
Scope
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces
cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.
This method is applicable to:
— fresh water;
— wastewater;
— aqueous extracts and leachates;
— eluates of sediments (fresh water);
— pore water;
— aqueous solutions of single substances or of chemical mixtures;
— drinking water.
The limit of quantification (LOQ) of this method for the direct analysis of water samples is between
8 ng/l and 15 ng/l 17β‐estradiol equivalents (EEQ) based on the results of the international
interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between
120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above
this threshold have to be diluted for a valid quantification. Extraction and pre‐concentration of water
samples can prove necessary, if their estrogenic potential is below the given LOQ.
