The method makes it possible to detect and identify spiramycin, tylosin and virginiamycin in animal feeding stuffs (feed raw materials of mainly plant origin and compound feeds) excluding mineral feeds and premixtures. The limit of detection is about 2 mg/kg for spiramycin, 1 mg/kg for tylosin and 1 mg/kg for virginiamycin. In some milk replacers, it can be slightly higher than 1 mg/kg for virginiamycin.
Note 1 : These limits of detection are probably little overestimated but were fully validated during the collaborative study (see annex B). In each laboratory, each day of analysis, spiked blank samples at 1 mg/kg for spiramycin and virginiamycin and at 0.5 mg/kg for tylosin are analysed for checking lower detection limits.
Some other antibiotics may interfere in the detection of these 3 specific macrolide antibiotics. The known interferences are specified in annex A of the method.
Registration number (WIID)
59408
Scope
The method makes it possible to detect and identify spiramycin, tylosin and virginiamycin in animal feeding stuffs (feed raw materials of mainly plant origin and compound feeds) excluding mineral feeds and premixtures. The limit of detection is about 2 mg/kg for spiramycin, 1 mg/kg for tylosin and 1 mg/kg for virginiamycin. In some milk replacers, it can be slightly higher than 1 mg/kg for virginiamycin.
Note 1 : These limits of detection are probably little overestimated but were fully validated during the collaborative study (see annex B). In each laboratory, each day of analysis, spiked blank samples at 1 mg/kg for spiramycin and virginiamycin and at 0.5 mg/kg for tylosin are analysed for checking lower detection limits.
Some other antibiotics may interfere in the detection of these 3 specific macrolide antibiotics. The known interferences are specified in annex A of the method.
