This European Standard specifies a high performance liquid chromatography – mass spectrometry (LC-MS/MS) method for the determination of tylosin, spiramycin, vriginiamycin, carbadox and olaquindox in animal feeds.
The method is suitable for the identification of low concentrations of tylosin, spiramycin, virginiamycin, carbadox and claquindox in compound animal feeds. The limit of detection of tylosin, spiramycin and virginiamycin is well below the target concentration of 1 mg/kg. The target concentrations of 4 mg/kg for carbadox and 3 mg/kg for olaquindox can also readily be achieved.
Identification of the target compounds according the EU requirements at the target concentration is possible.
Since tylosin, spiramycin and virginiamycin are fermentation products consisting of a mixture of several closely related compounds, the analysis is based on detection and identification of the most abundant constituents. For tylosin the marker is tylosin A, for spiramycin that is spiramycin I and II and for virginamycin that is virginiamycin M1 and S1. The other isomers and forms can be readily detected with the same method but adjustment of the MS parameters according to the molecular mass of precursor and product ions need to be made. Carbadox and Olaquindox are analysed as such.
Quantification of the content is performed by multi-level standard addition to account for the considerable variability of feed composition. (Annex B)
Registration number (WIID)
59404
Scope
This European Standard specifies a high performance liquid chromatography – mass spectrometry (LC-MS/MS) method for the determination of tylosin, spiramycin, vriginiamycin, carbadox and olaquindox in animal feeds.
The method is suitable for the identification of low concentrations of tylosin, spiramycin, virginiamycin, carbadox and claquindox in compound animal feeds. The limit of detection of tylosin, spiramycin and virginiamycin is well below the target concentration of 1 mg/kg. The target concentrations of 4 mg/kg for carbadox and 3 mg/kg for olaquindox can also readily be achieved.
Identification of the target compounds according the EU requirements at the target concentration is possible.
Since tylosin, spiramycin and virginiamycin are fermentation products consisting of a mixture of several closely related compounds, the analysis is based on detection and identification of the most abundant constituents. For tylosin the marker is tylosin A, for spiramycin that is spiramycin I and II and for virginamycin that is virginiamycin M1 and S1. The other isomers and forms can be readily detected with the same method but adjustment of the MS parameters according to the molecular mass of precursor and product ions need to be made. Carbadox and Olaquindox are analysed as such.
Quantification of the content is performed by multi-level standard addition to account for the considerable variability of feed composition. (Annex B)
