<p>ISO 14892|IDF 177:2002 specifies a method for the determination of vitamin D in a test sample containing at least 10 µg of vitamin D per 100 g [equal to 400 International Units (IU) of vitamin D per 100 g] by using high performance liquid chromatography (HPLC).</p>
<p>The results of the determination are only reliable if, in the case of determination of vitamin D<sub>3</sub>, the test sample contains only vitamin D<sub>3</sub>, and no vitamin D<sub>2</sub> (which will be added as an internal standard) and, in the case of determination of vitamin D<sub>2</sub>, the test sample contains only vitamin D<sub>2</sub> and no vitamin D<sub>3</sub> (which will be added as an internal standard). This is to be verified by the procedure carried out without the addition of the internal standard (vitamin D<sub>2</sub>).</p>
Registration number (WIID)
25872
Scope
<p>ISO 14892|IDF 177:2002 specifies a method for the determination of vitamin D in a test sample containing at least 10 µg of vitamin D per 100 g [equal to 400 International Units (IU) of vitamin D per 100 g] by using high performance liquid chromatography (HPLC).</p>
<p>The results of the determination are only reliable if, in the case of determination of vitamin D<sub>3</sub>, the test sample contains only vitamin D<sub>3</sub>, and no vitamin D<sub>2</sub> (which will be added as an internal standard) and, in the case of determination of vitamin D<sub>2</sub>, the test sample contains only vitamin D<sub>2</sub> and no vitamin D<sub>3</sub> (which will be added as an internal standard). This is to be verified by the procedure carried out without the addition of the internal standard (vitamin D<sub>2</sub>).</p>
