<P>ISO 14892|IDF 177:2002 specifies a method for the determination of vitamin D in a test sample containing at least 10 µg of vitamin D per 100 g [equal to 400 International Units (IU) of vitamin D per 100 g] by using high performance liquid chromatography (HPLC).</P>
<P>The results of the determination are only reliable if, in the case of determination of vitamin D<SUB>3</SUB>, the test sample contains only vitamin D<SUB>3</SUB>, and no vitamin D<SUB>2</SUB> (which will be added as an internal standard) and, in the case of determination of vitamin D<SUB>2</SUB>, the test sample contains only vitamin D<SUB>2</SUB> and no vitamin D<SUB>3</SUB> (which will be added as an internal standard). This is to be verified by the procedure carried out without the addition of the internal standard (vitamin D<SUB>2</SUB>).</P>
Registration number (WIID)
25872
Scope
<P>ISO 14892|IDF 177:2002 specifies a method for the determination of vitamin D in a test sample containing at least 10 µg of vitamin D per 100 g [equal to 400 International Units (IU) of vitamin D per 100 g] by using high performance liquid chromatography (HPLC).</P>
<P>The results of the determination are only reliable if, in the case of determination of vitamin D<SUB>3</SUB>, the test sample contains only vitamin D<SUB>3</SUB>, and no vitamin D<SUB>2</SUB> (which will be added as an internal standard) and, in the case of determination of vitamin D<SUB>2</SUB>, the test sample contains only vitamin D<SUB>2</SUB> and no vitamin D<SUB>3</SUB> (which will be added as an internal standard). This is to be verified by the procedure carried out without the addition of the internal standard (vitamin D<SUB>2</SUB>).</P>
