ISO/TC 34/SC 5

Project No.ISO 29981:2024
Title<p class="MsoBodyText"><span lang="EN-GB">This document specifies a method for the selective enumeration of bifidobacteria in milk products by using a colony-count technique at 37 °C under anaerobic conditions.</span></p> <p class="MsoBodyText"><span lang="EN-GB">The method is applicable to milk products, such as fermented (e.g. yoghurts) and non-fermented milks (e.g. pasteurized milks, skim milks, whey protein concentrates), milk powders and formulae (e.g. infant formulae, follow-up formulae for older infants, products for young children) where these microorganisms are present and viable, in combination with other lactic acid bacteria or alone. The method is also applicable to starter and probiotic cultures. For proposed quality criteria of dairy products, see, for example, CXS 243-2003.</span></p> <p class="MsoBodyText"><span lang="EN-GB">Bifidobacteria used in milk products usually belong to the following species (e.g. References [7] and [10]):</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">Bifidobacterium adolescentis</span></em><span lang="EN-GB">;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. animalis</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">animalis</em>;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. animalis</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">lactis</em>;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. bifidum</span></em><span lang="EN-GB">;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. breve</span></em><span lang="EN-GB">;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. longum</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">infantis</em>;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. longum</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">longum</em>.</span></p>
Registration number (WIID)79391
Scope<p class="MsoBodyText"><span lang="EN-GB">This document specifies a method for the selective enumeration of bifidobacteria in milk products by using a colony-count technique at 37 °C under anaerobic conditions.</span></p> <p class="MsoBodyText"><span lang="EN-GB">The method is applicable to milk products, such as fermented (e.g. yoghurts) and non-fermented milks (e.g. pasteurized milks, skim milks, whey protein concentrates), milk powders and formulae (e.g. infant formulae, follow-up formulae for older infants, products for young children) where these microorganisms are present and viable, in combination with other lactic acid bacteria or alone. The method is also applicable to starter and probiotic cultures. For proposed quality criteria of dairy products, see, for example, CXS 243-2003.</span></p> <p class="MsoBodyText"><span lang="EN-GB">Bifidobacteria used in milk products usually belong to the following species (e.g. References [7] and [10]):</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">Bifidobacterium adolescentis</span></em><span lang="EN-GB">;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. animalis</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">animalis</em>;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. animalis</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">lactis</em>;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. bifidum</span></em><span lang="EN-GB">;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. breve</span></em><span lang="EN-GB">;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. longum</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">infantis</em>;</span></p> <p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';">     </span></span></span><!--[endif]--><em style="mso-bidi-font-style: normal;"><span lang="EN-GB">B. longum</span></em><span lang="EN-GB"> subsp. <em style="mso-bidi-font-style: normal;">longum</em>.</span></p>
StatusStandarts spēkā
ICS group67.100.01