<p>ISO/TS 12869:2012 specifies a method for the detection and quantification of <i>Legionella</i> spp. and <i>L. pneumophila</i> using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.</p>
<p>Technical details specified in ISO/TS 12869:2012 are given for information only. Any other technical solutions complying with the performance requirements are suitable.</p>
<p>ISO/TS 12869:2012 is intended to be applied in the bacteriological investigation of all types of water (both hot and cold), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.</p>
<p>The results are expressed as the number of genome units of <i>Legionella</i> spp. and/or <i>L. pneumophila</i> per litre of sample.</p>
<p>The method described in ISO/TS 12869:2012 is applicable to all types of water. However, some additives, e.g. chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.</p>
<p>The qPCR methods do not give any information about live or dead cells.</p>
Registration number (WIID)
52079
Scope
<p>ISO/TS 12869:2012 specifies a method for the detection and quantification of <i>Legionella</i> spp. and <i>L. pneumophila</i> using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.</p>
<p>Technical details specified in ISO/TS 12869:2012 are given for information only. Any other technical solutions complying with the performance requirements are suitable.</p>
<p>ISO/TS 12869:2012 is intended to be applied in the bacteriological investigation of all types of water (both hot and cold), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.</p>
<p>The results are expressed as the number of genome units of <i>Legionella</i> spp. and/or <i>L. pneumophila</i> per litre of sample.</p>
<p>The method described in ISO/TS 12869:2012 is applicable to all types of water. However, some additives, e.g. chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.</p>
<p>The qPCR methods do not give any information about live or dead cells.</p>
