<p>This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.</p>
<p>Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable.</p>
<p>NOTE 1 For performance requirements, see Clause 9.</p>
<p>This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.</p>
<p>NOTE 2 For validation requirements, see 9.7.</p>
<p>The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.</p>
<p>The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.</p>
<p>The qPCR methods do not give any information about the physiological state of the Legionella.</p>
Registration number (WIID)
70756
Scope
<p>This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.</p>
<p>Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable.</p>
<p>NOTE 1 For performance requirements, see Clause 9.</p>
<p>This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.</p>
<p>NOTE 2 For validation requirements, see 9.7.</p>
<p>The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.</p>
<p>The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.</p>
<p>The qPCR methods do not give any information about the physiological state of the Legionella.</p>
