<p>This International Standard specifies five methods for determining the ultimate aerobic biodegradability of organic</p>
<p>compounds in the marine environment by aerobic microorganisms in static aqueous test systems. Standard</p>
<p>degradation methods developed for testing in fresh water are modified and adapted to marine conditions. These</p>
<p>methods are the DOC die-away test (ISO 7827), the closed bottle test (ISO 10707), the two-phase closed bottle</p>
<p>test (ISO 10708), the CO2 evolution test (ISO 9439) and the CO2 headspace test (ISO 14593).</p>
<p>The methods apply to organic compounds which</p>
<p>a) are water-soluble under the conditions of the test used;</p>
<p>b) are poorly water-soluble under the conditions of the test used, in which case special measures may be</p>
<p>necessary to achieve good dispersion of the compound (see for example, ISO 10634);</p>
<p>c) are volatile, provided that an appropriate test with suitable conditions is used;</p>
<p>d) are not inhibitory to the test microorganisms at the concentration chosen for the tests. The presence of</p>
<p>inhibitory effects can be determined as specified in this International Standard.</p>
<p>NOTE The conditions described in this International Standard do not always correspond to the optimal conditions for</p>
<p>allowing the maximum degree of biodegradation to occur. For biodegradation methods in fresh water see ISO 14593 and</p>
<p>ISO 15462, and for biodegradation at low concentrations see ISO 14592.</p>
Registration number (WIID)
30205
Scope
<p>This International Standard specifies five methods for determining the ultimate aerobic biodegradability of organic</p>
<p>compounds in the marine environment by aerobic microorganisms in static aqueous test systems. Standard</p>
<p>degradation methods developed for testing in fresh water are modified and adapted to marine conditions. These</p>
<p>methods are the DOC die-away test (ISO 7827), the closed bottle test (ISO 10707), the two-phase closed bottle</p>
<p>test (ISO 10708), the CO2 evolution test (ISO 9439) and the CO2 headspace test (ISO 14593).</p>
<p>The methods apply to organic compounds which</p>
<p>a) are water-soluble under the conditions of the test used;</p>
<p>b) are poorly water-soluble under the conditions of the test used, in which case special measures may be</p>
<p>necessary to achieve good dispersion of the compound (see for example, ISO 10634);</p>
<p>c) are volatile, provided that an appropriate test with suitable conditions is used;</p>
<p>d) are not inhibitory to the test microorganisms at the concentration chosen for the tests. The presence of</p>
<p>inhibitory effects can be determined as specified in this International Standard.</p>
<p>NOTE The conditions described in this International Standard do not always correspond to the optimal conditions for</p>
<p>allowing the maximum degree of biodegradation to occur. For biodegradation methods in fresh water see ISO 14593 and</p>
<p>ISO 15462, and for biodegradation at low concentrations see ISO 14592.</p>