<p>This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and</p>
<p>wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio</p>
<p>(Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may</p>
<p>include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with</p>
<p>ISO 7346-1, ISO 7346-2 or ISO 7346-3.</p>
<p>The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test</p>
<p>conditions are made, particularly with regard to the temperature and volume per fish biomass.</p>
Registration number (WIID)
2171
Scope
<p>This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and</p>
<p>wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio</p>
<p>(Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may</p>
<p>include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with</p>
<p>ISO 7346-1, ISO 7346-2 or ISO 7346-3.</p>
<p>The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test</p>
<p>conditions are made, particularly with regard to the temperature and volume per fish biomass.</p>
