<p>This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha.</p>
<p>Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %.</p>
<p>This method is applicable to:</p>
<p>— fresh water;</p>
<p>— waste water;</p>
<p>— sea water;</p>
<p>— brackish water;</p>
<p>— aqueous extracts and leachates;</p>
<p>— eluates of sediments (fresh water);</p>
<p>— pore water;</p>
<p>— aqueous solutions of single substances or of chemical mixtures;</p>
<p>— drinking water.</p>
<p>The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng/l and 3 ng/l 17β-estradiol equivalents (EEQ). The upper threshold of the dynamic range for this test is between 25 ng/l and 40 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.</p>
<p>An international interlaboratory trial for the validation of this document has been carried out. The results are summarized in Annex F.</p>
<p>NOTE Extraction and pre-concentration of water samples can prove necessary.</p>
Registration number (WIID)
64451
Scope
<p>This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha.</p>
<p>Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %.</p>
<p>This method is applicable to:</p>
<p>— fresh water;</p>
<p>— waste water;</p>
<p>— sea water;</p>
<p>— brackish water;</p>
<p>— aqueous extracts and leachates;</p>
<p>— eluates of sediments (fresh water);</p>
<p>— pore water;</p>
<p>— aqueous solutions of single substances or of chemical mixtures;</p>
<p>— drinking water.</p>
<p>The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng/l and 3 ng/l 17β-estradiol equivalents (EEQ). The upper threshold of the dynamic range for this test is between 25 ng/l and 40 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.</p>
<p>An international interlaboratory trial for the validation of this document has been carried out. The results are summarized in Annex F.</p>
<p>NOTE Extraction and pre-concentration of water samples can prove necessary.</p>
