This document specifies a method for the determination of the estrogen activity of water and waste water by means of a reporter gene assay with genetically modified Oryzias latipes (O. latipes, medaka) eleutheroembryos.
This method is applicable to:
- fresh water;
- waste water;
- aqueous extracts and leachates;
- eluates of sediments (fresh water);
- pore water;
- aqueous solutions of single substances or of chemical mixtures;
- drinking water.
NOTE 1: Extraction, pre-concentration or dilution of water samples can prove necessary
The organism preparation methods (freeze-drying, extraction, mineralization) and quantification of the chemical substances do not fall within the scope of this document.
Registration number (WIID)
86839
Scope
This document specifies a method for the determination of the estrogen activity of water and waste water by means of a reporter gene assay with genetically modified Oryzias latipes (O. latipes, medaka) eleutheroembryos.
This method is applicable to:
- fresh water;
- waste water;
- aqueous extracts and leachates;
- eluates of sediments (fresh water);
- pore water;
- aqueous solutions of single substances or of chemical mixtures;
- drinking water.
NOTE 1: Extraction, pre-concentration or dilution of water samples can prove necessary
The organism preparation methods (freeze-drying, extraction, mineralization) and quantification of the chemical substances do not fall within the scope of this document.
