This document specifies a method with the minimum technical requirements for DNA extraction of diatom samples, for use in DNA-based approaches dedicated to taxonomic identification of diatom communities, as well as ecological status and water quality assessments. The objective is to ensure high-quality DNA yields suitable for the subsequent molecular steps of DNA-based approaches (e.g. DNA metabarcoding). This document does not aim to cover the PCR, library preparation, sequencing, and data analysis steps of the workflow, which will be given in separate standards or technical reports.
Registration number (WIID)
94013
Scope
This document specifies a method with the minimum technical requirements for DNA extraction of diatom samples, for use in DNA-based approaches dedicated to taxonomic identification of diatom communities, as well as ecological status and water quality assessments. The objective is to ensure high-quality DNA yields suitable for the subsequent molecular steps of DNA-based approaches (e.g. DNA metabarcoding). This document does not aim to cover the PCR, library preparation, sequencing, and data analysis steps of the workflow, which will be given in separate standards or technical reports.
