<p>ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic bioserotypes of <i>Y. enterocolitica</i> and one for detection of <i>Y. pseudotuberculosis</i> by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. <i>Y. pestis</i>, the causative agent of bubonic and pneumonic plague harbours a variant of the <i>ail</i> gene as well and will be detected by the same primer/probe set as <i>Y. pseudotuberculosis</i>. However, <i>Y. pestis</i> is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.</p>
Registration number (WIID)
63635
Scope
<p>ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic bioserotypes of <i>Y. enterocolitica</i> and one for detection of <i>Y. pseudotuberculosis</i> by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. <i>Y. pestis</i>, the causative agent of bubonic and pneumonic plague harbours a variant of the <i>ail</i> gene as well and will be detected by the same primer/probe set as <i>Y. pseudotuberculosis</i>. However, <i>Y. pestis</i> is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.</p>
