<p class="MsoBodyText"><span lang="EN-GB">This document specifies the detection of <em style="mso-bidi-font-style: normal;">Clostridium </em>(<em style="mso-bidi-font-style: normal;">C.</em>)<em style="mso-bidi-font-style: normal;"> perfringens</em>.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This document is applicable to:</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">products intended for human consumption;</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">products intended for animal feeding;</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">environmental samples in the area of food and feed production and handling;</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">samples from the primary production stage.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.</span></p>
<p class="Note"><span lang="EN-GB">NOTE<span style="mso-tab-count: 1;"> </span>Interlaboratory studies with a small number of participating laboratories (<10) were conducted for the following food categories:</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">ready-to-eat, ready-to-reheat meat products;</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">eggs and egg products (derivates);</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">ready-to-eat, ready-to-reheat fishery products;</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">processed fruits and vegetables;</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">infant formula and infant cereals (with probiotics);</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">multi-component foods or meal components.</span></p>
<p class="Note"><span lang="EN-GB">It has also been validated with a small number of participating laboratories for the following other category:</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">environmental samples (food or feed production).</span></p>
<p class="Note"><span lang="EN-GB">Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. The method has not been validated for the category ‘pet food and animal feed’, as the test samples used for the interlaboratory study were already naturally contaminated with <em style="mso-bidi-font-style: normal;">C. perfringens</em>. Given the limited number of participating laboratories in the interlaboratory studies, the calculated performance characteristics can be used as indicative values of the method performance. For detailed information on the validation, see Clause 11 and Annexes C to F.</span></p>
Registration number (WIID)
71499
Scope
<p class="MsoBodyText"><span lang="EN-GB">This document specifies the detection of <em style="mso-bidi-font-style: normal;">Clostridium </em>(<em style="mso-bidi-font-style: normal;">C.</em>)<em style="mso-bidi-font-style: normal;"> perfringens</em>.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This document is applicable to:</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">products intended for human consumption;</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">products intended for animal feeding;</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">environmental samples in the area of food and feed production and handling;</span></p>
<p class="ListContinue1" style="mso-list: l0 level1 lfo1;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">samples from the primary production stage.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.</span></p>
<p class="Note"><span lang="EN-GB">NOTE<span style="mso-tab-count: 1;"> </span>Interlaboratory studies with a small number of participating laboratories (<10) were conducted for the following food categories:</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">ready-to-eat, ready-to-reheat meat products;</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">eggs and egg products (derivates);</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">ready-to-eat, ready-to-reheat fishery products;</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">processed fruits and vegetables;</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">infant formula and infant cereals (with probiotics);</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">multi-component foods or meal components.</span></p>
<p class="Note"><span lang="EN-GB">It has also been validated with a small number of participating laboratories for the following other category:</span></p>
<p class="ListContinue1-" style="margin-left: 18.0pt; text-indent: -18.0pt; mso-list: l1 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB" style="mso-fareast-font-family: Cambria; mso-bidi-font-family: Cambria;"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">environmental samples (food or feed production).</span></p>
<p class="Note"><span lang="EN-GB">Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. The method has not been validated for the category ‘pet food and animal feed’, as the test samples used for the interlaboratory study were already naturally contaminated with <em style="mso-bidi-font-style: normal;">C. perfringens</em>. Given the limited number of participating laboratories in the interlaboratory studies, the calculated performance characteristics can be used as indicative values of the method performance. For detailed information on the validation, see Clause 11 and Annexes C to F.</span></p>
