<p>ISO 12966-2:2011 specifies methods of preparing the methyl esters of fatty acids.</p>
<p>It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF<sub>3</sub> transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides.</p>
<p>Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GC), thin-layer chromatography (TLC), and infrared spectrometry (IR).</p>
Registration number (WIID)
43172
Scope
<p>ISO 12966-2:2011 specifies methods of preparing the methyl esters of fatty acids.</p>
<p>It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF<sub>3</sub> transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides.</p>
<p>Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GC), thin-layer chromatography (TLC), and infrared spectrometry (IR).</p>
