This TS specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The methods detect the two pathogens by PCR and allow isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbour a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This TS is applicable to products for human consumption, animal feeding stuffs and environmental samples.
Registration number (WIID)
40275
Scope
This TS specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The methods detect the two pathogens by PCR and allow isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbour a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This TS is applicable to products for human consumption, animal feeding stuffs and environmental samples.
