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<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">This document specifies a procedure for the detection of a DNA sequence of the open reading frame five (ORF V) from cauliflower mosaic virus (CaMV) and a procedure for the detection of the DNA sequence of the nopaline synthase (<em style="mso-bidi-font-style: normal;">nos</em>) gene from tumour-inducing (Ti) plasmids of phytopathogenic <em style="mso-bidi-font-style: normal;">Rhizobium radiobacter</em> (formerly named <em style="mso-bidi-font-style: normal;">Agrobacterium tumefaciens</em>). The procedures can be used in the context of screening for genetically modified crop/plants and their derived products to further clarify a positive PCR result for a specific promoter or terminator of CaMV (P-35S, T-35S), or both, and the <em style="mso-bidi-font-style: normal;">nos</em> gene (P-<em style="mso-bidi-font-style: normal;">nos</em>, T-<em style="mso-bidi-font-style: normal;">nos</em>), respectively.</span></p>
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">The methods specified in this document will detect and identify naturally occurring CaMV or <em style="mso-bidi-font-style: normal;">Rhizobium radiobacter</em> (Ti plasmid) DNA, or both, if present in the sample in the absence of a genetically modified plant event containing the specified target sequences.</span></p>
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">Both methods are based on the real-time polymerase chain reaction (PCR) and are applicable for the analysis of DNA extracted from foodstuffs and other products such as feedstuffs and seeds/grains. The application of the methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.</span></p>
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">With appropriate calibration material, the CaMV ORF V or <em style="mso-bidi-font-style: normal;">nos</em> copy number, or both, can be estimated and compared, respectively, with the estimated copy number for the promoter (P-35S, P-<em style="mso-bidi-font-style: normal;">nos</em>) or the terminator (T-35S, T-<em style="mso-bidi-font-style: normal;">nos</em>) sequences, or both. Thereby, conclusions are possible about the presence of an unknown genetically modified organism (GMO) in addition to any detected CaMV DNA or <em style="mso-bidi-font-style: normal;">Rhizobium radiobacter</em> Ti plasmid DNA, or both, in a test sample.</span></p>
Reģistrācijas numurs (WIID)
84434
Darbības sfēra
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">This document specifies a procedure for the detection of a DNA sequence of the open reading frame five (ORF V) from cauliflower mosaic virus (CaMV) and a procedure for the detection of the DNA sequence of the nopaline synthase (<em style="mso-bidi-font-style: normal;">nos</em>) gene from tumour-inducing (Ti) plasmids of phytopathogenic <em style="mso-bidi-font-style: normal;">Rhizobium radiobacter</em> (formerly named <em style="mso-bidi-font-style: normal;">Agrobacterium tumefaciens</em>). The procedures can be used in the context of screening for genetically modified crop/plants and their derived products to further clarify a positive PCR result for a specific promoter or terminator of CaMV (P-35S, T-35S), or both, and the <em style="mso-bidi-font-style: normal;">nos</em> gene (P-<em style="mso-bidi-font-style: normal;">nos</em>, T-<em style="mso-bidi-font-style: normal;">nos</em>), respectively.</span></p>
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">The methods specified in this document will detect and identify naturally occurring CaMV or <em style="mso-bidi-font-style: normal;">Rhizobium radiobacter</em> (Ti plasmid) DNA, or both, if present in the sample in the absence of a genetically modified plant event containing the specified target sequences.</span></p>
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">Both methods are based on the real-time polymerase chain reaction (PCR) and are applicable for the analysis of DNA extracted from foodstuffs and other products such as feedstuffs and seeds/grains. The application of the methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.</span></p>
<p class="MsoBodyText" style="mso-layout-grid-align: none; text-autospace: none;"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">With appropriate calibration material, the CaMV ORF V or <em style="mso-bidi-font-style: normal;">nos</em> copy number, or both, can be estimated and compared, respectively, with the estimated copy number for the promoter (P-35S, P-<em style="mso-bidi-font-style: normal;">nos</em>) or the terminator (T-35S, T-<em style="mso-bidi-font-style: normal;">nos</em>) sequences, or both. Thereby, conclusions are possible about the presence of an unknown genetically modified organism (GMO) in addition to any detected CaMV DNA or <em style="mso-bidi-font-style: normal;">Rhizobium radiobacter</em> Ti plasmid DNA, or both, in a test sample.</span></p>
