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<p class="MsoBodyText"><span lang="EN-GB">This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of pigeon-specific DNA derived from food and feed. The method can be applied to distinguish rock pigeon (<em style="mso-bidi-font-style: normal;">Columba livia</em>) from other domestic poultry meats (e. g. goose, duck, quail, pheasant). It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">The target sequence is a partial fragment of <em style="mso-bidi-font-style: normal;">Columba livia</em> breed Danish Tumbler unplaced genomic scaffold, Cliv_1.0 scaffold114 (i.e. GenBank accession number NW_004973337.1),<sup>[</sup><sup>1</sup><sup>]</sup> which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD<sub>95 %</sub>).</span></p>
Reģistrācijas numurs (WIID)
86175
Darbības sfēra
<p class="MsoBodyText"><span lang="EN-GB">This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of pigeon-specific DNA derived from food and feed. The method can be applied to distinguish rock pigeon (<em style="mso-bidi-font-style: normal;">Columba livia</em>) from other domestic poultry meats (e. g. goose, duck, quail, pheasant). It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">The target sequence is a partial fragment of <em style="mso-bidi-font-style: normal;">Columba livia</em> breed Danish Tumbler unplaced genomic scaffold, Cliv_1.0 scaffold114 (i.e. GenBank accession number NW_004973337.1),<sup>[</sup><sup>1</sup><sup>]</sup> which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD<sub>95 %</sub>).</span></p>
