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<p class="MsoBodyText"><span lang="NL" style="mso-ansi-language: NL;">This document specifies a method for the enumeration of intestinal enterococci in water, including <em style="mso-bidi-font-style: normal;">Enterococcus faecalis</em>, <em style="mso-bidi-font-style: normal;">Enterococcus faecium</em>, <em style="mso-bidi-font-style: normal;">Enterococcus durans</em>, <em style="mso-bidi-font-style: normal;">Enterococcus avium</em>, <em style="mso-bidi-font-style: normal;">Enterococcus gallinarum</em>, <em style="mso-bidi-font-style: normal;">Enterococcus hirae</em>, <em style="mso-bidi-font-style: normal;">Enterococcus casselifavus</em>. </span><span lang="EN-GB">The method is based on the growth of target organisms in a liquid medium and calculation of the “most probable number” (MPN) of microorganisms by reference to MPN tables or using suitable MPN informatic programs.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This method can be applied to drinking water and bathing water (fresh or marine), together with other similar water types including those containing an appreciable amount of suspended matter, and allows the detection of enterococci at 1 colony-forming unit (CFU) per 100 ml with definitive results within (26 ± 2) h in the presence of heterotrophic bacteria in numbers as high as 1 × 10<sup>6</sup> per 100 ml of sample.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">For bathing waters, fresh and marine, enterococci are best enumerated when samples are diluted 1:10.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">The test specified in this document relies upon the detection of intestinal enterococci based upon expression of the enzyme ß-D-glucosidase and provides a confirmed result in 24 h without further testing of positive wells.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This document does not apply to bottled waters, for which the method has not been validated and therefore is outside the scope of this document, unless appropriate validation of performance of this method has been undertaken by the laboratory prior to use.</span></p>
Reģistrācijas numurs (WIID)
85093
Darbības sfēra
<p class="MsoBodyText"><span lang="NL" style="mso-ansi-language: NL;">This document specifies a method for the enumeration of intestinal enterococci in water, including <em style="mso-bidi-font-style: normal;">Enterococcus faecalis</em>, <em style="mso-bidi-font-style: normal;">Enterococcus faecium</em>, <em style="mso-bidi-font-style: normal;">Enterococcus durans</em>, <em style="mso-bidi-font-style: normal;">Enterococcus avium</em>, <em style="mso-bidi-font-style: normal;">Enterococcus gallinarum</em>, <em style="mso-bidi-font-style: normal;">Enterococcus hirae</em>, <em style="mso-bidi-font-style: normal;">Enterococcus casselifavus</em>. </span><span lang="EN-GB">The method is based on the growth of target organisms in a liquid medium and calculation of the “most probable number” (MPN) of microorganisms by reference to MPN tables or using suitable MPN informatic programs.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This method can be applied to drinking water and bathing water (fresh or marine), together with other similar water types including those containing an appreciable amount of suspended matter, and allows the detection of enterococci at 1 colony-forming unit (CFU) per 100 ml with definitive results within (26 ± 2) h in the presence of heterotrophic bacteria in numbers as high as 1 × 10<sup>6</sup> per 100 ml of sample.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">For bathing waters, fresh and marine, enterococci are best enumerated when samples are diluted 1:10.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">The test specified in this document relies upon the detection of intestinal enterococci based upon expression of the enzyme ß-D-glucosidase and provides a confirmed result in 24 h without further testing of positive wells.</span></p>
<p class="MsoBodyText"><span lang="EN-GB">This document does not apply to bottled waters, for which the method has not been validated and therefore is outside the scope of this document, unless appropriate validation of performance of this method has been undertaken by the laboratory prior to use.</span></p>
