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<p>This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.</p>
<p>This method is applicable to:</p>
<p>— fresh water;</p>
<p>— waste water;</p>
<p>— aqueous extracts and leachates;</p>
<p>— eluates of sediments (fresh water);</p>
<p>— pore water;</p>
<p>— aqueous solutions of single substances or of chemical mixtures;</p>
<p>— drinking water.</p>
<p>The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.</p>
Reģistrācijas numurs (WIID)
64450
Darbības sfēra
<p>This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.</p>
<p>This method is applicable to:</p>
<p>— fresh water;</p>
<p>— waste water;</p>
<p>— aqueous extracts and leachates;</p>
<p>— eluates of sediments (fresh water);</p>
<p>— pore water;</p>
<p>— aqueous solutions of single substances or of chemical mixtures;</p>
<p>— drinking water.</p>
<p>The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.</p>
