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<p class="MsoNormal"><span lang="EN-GB">This document specifies a method for the determination of the dioxin‑like activity of water and wastewater by means of an AhR‑mediated reporter gene assay utilizing stably transfected mammalian cell line. This reporter gene assay is based on the activation of the mammalian AhR signalling pathway.</span></p>
<p class="MsoNormal"><span lang="EN-GB">This method is applicable to:</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">drinking water;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">fresh water;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">groundwater;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB" style="mso-fareast-language: JA;">river water;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">wastewater.</span></p>
<p class="MsoNormal"><span lang="EN-GB">The limit of quantification (LOQ) at <em style="mso-bidi-font-style: normal;">CV</em> 20 % or less of this method is 0,5 pg and 1,0 pg 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (2,3,7,8-TCDD) equivalents (TCDD-EQ)/l based on the results of laboratory trials using appropriately extracted and cleaned up water samples for dioxins and dioxin‑like compounds in rat and mouse cell lines, respectively. The LOQ depends on the sample volume. For determination of TCDD-EQ derived from dioxins and dioxin‑like compounds in water and wastewater, suitable extraction and clean‑up are necessary for water samples, since AhR ligands/agonists are composed of a wide variety of structurally diverse compounds including natural, and anthropology-natural substances.</span></p>
<p class="Note"><span lang="EN-GB" style="mso-fareast-language: JA;">NOTE<span style="mso-tab-count: 1;"> </span>In this document, TCDD-EQ is used instead of TEQ which represents toxicity equivalent quantity.</span></p>
Reģistrācijas numurs (WIID)
86910
Darbības sfēra
<p class="MsoNormal"><span lang="EN-GB">This document specifies a method for the determination of the dioxin‑like activity of water and wastewater by means of an AhR‑mediated reporter gene assay utilizing stably transfected mammalian cell line. This reporter gene assay is based on the activation of the mammalian AhR signalling pathway.</span></p>
<p class="MsoNormal"><span lang="EN-GB">This method is applicable to:</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">drinking water;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">fresh water;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">groundwater;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB" style="mso-fareast-language: JA;">river water;</span></p>
<p class="MsoListContinue" style="mso-list: l0 level1 lfo2;"><!-- [if !supportLists]--><span lang="EN-GB"><span style="mso-list: Ignore;">—<span style="font: 7.0pt 'Times New Roman';"> </span></span></span><!--[endif]--><span lang="EN-GB">wastewater.</span></p>
<p class="MsoNormal"><span lang="EN-GB">The limit of quantification (LOQ) at <em style="mso-bidi-font-style: normal;">CV</em> 20 % or less of this method is 0,5 pg and 1,0 pg 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (2,3,7,8-TCDD) equivalents (TCDD-EQ)/l based on the results of laboratory trials using appropriately extracted and cleaned up water samples for dioxins and dioxin‑like compounds in rat and mouse cell lines, respectively. The LOQ depends on the sample volume. For determination of TCDD-EQ derived from dioxins and dioxin‑like compounds in water and wastewater, suitable extraction and clean‑up are necessary for water samples, since AhR ligands/agonists are composed of a wide variety of structurally diverse compounds including natural, and anthropology-natural substances.</span></p>
<p class="Note"><span lang="EN-GB" style="mso-fareast-language: JA;">NOTE<span style="mso-tab-count: 1;"> </span>In this document, TCDD-EQ is used instead of TEQ which represents toxicity equivalent quantity.</span></p>
