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<p>This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the</p>
<p>global structure and the abundance of soil bacterial communities using PCR-based technologies. This method</p>
<p>is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in</p>
<p>organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.</p>
<p>The direct extraction of DNA from soil samples provides unique insight into the richness and structure of</p>
<p>microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular</p>
<p>approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising</p>
<p>domain and can contribute in the near future to the development of routine tools to monitor the microbiota of</p>
<p>soil environments.</p>
<p>Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved</p>
<p>thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a</p>
<p>result, traces of plant DNA can contaminate the soil DNA extract.</p>
Reģistrācijas numurs (WIID)
50025
Darbības sfēra
<p>This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the</p>
<p>global structure and the abundance of soil bacterial communities using PCR-based technologies. This method</p>
<p>is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in</p>
<p>organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.</p>
<p>The direct extraction of DNA from soil samples provides unique insight into the richness and structure of</p>
<p>microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular</p>
<p>approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising</p>
<p>domain and can contribute in the near future to the development of routine tools to monitor the microbiota of</p>
<p>soil environments.</p>
<p>Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved</p>
<p>thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a</p>
<p>result, traces of plant DNA can contaminate the soil DNA extract.</p>
