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<p>This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:</p>
<p>a) handling of bacterial cultures;</p>
<p>b) axenic genomic DNA isolation;</p>
<p>c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;</p>
<p>d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;</p>
<p>e) metadata capture and sequence repository deposition;</p>
<p>f) validation of the end-to-end WGS workflow (fit for purpose for intended application).</p>
<p>This document is applicable to bacteria isolated from:</p>
<p>— products intended for human consumption;</p>
<p>— products intended for animal feed;</p>
<p>— environmental samples from food and feed handling and production areas;</p>
<p>— samples from the primary production stage.</p>
Reģistrācijas numurs (WIID)
75509
Darbības sfēra
<p>This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:</p>
<p>a) handling of bacterial cultures;</p>
<p>b) axenic genomic DNA isolation;</p>
<p>c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;</p>
<p>d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;</p>
<p>e) metadata capture and sequence repository deposition;</p>
<p>f) validation of the end-to-end WGS workflow (fit for purpose for intended application).</p>
<p>This document is applicable to bacteria isolated from:</p>
<p>— products intended for human consumption;</p>
<p>— products intended for animal feed;</p>
<p>— environmental samples from food and feed handling and production areas;</p>
<p>— samples from the primary production stage.</p>
